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This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Never before has it been so critical for lab workers to possess the proper tools and methodologies necessary to determine the structure, function, and expression of the corresponding proteins encoded in the genome. Mulhardt's Molecular Biology and Genomics helps aid in this daunting task by providing the reader with tips and tricks for more successful lab experiments. This strategic lab guide explores the current methodological variety of molecular biology and genomics in a simple manner, addressing the assets and drawbacks as well as critical points. It also provides short and precise summaries of routine procedures as well as listings of the advantages and disadvantages of alternative methods. * Shows how to avoid experimental dead ends and develops an instinct for the right experiment at the right time * Includes a handy Career Guide for researchers in the field * Contains more than 100 extensive figures and tables
The goal of this book is to make it easier for scientists, especially those new to scientific writing, to write about their results and to get their manuscripts accepted in peer-reviewed journals. The book covers each step throughout the submission process, from organizing and outlining the manuscript, presenting statistical data and results, to what happens during the in-house manuscript review process and what to do if an article is initially rejected. In addition to providing practical exercises on these topics, the book focuses on helping writers distil their research into concise take-home messages for readers, in order to convey information as clearly as possible to the target audience.